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Eighteen differential metabolites were identified, with positive correlations found with 1-Palmitoylphosphatidylcholine, PC(16:0/18:3(9Z,12Z,15Z)), PE(15:0/22:1(13Z)), PE(22:6(4Z,7Z,10Z,13Z,16Z,19Z)/22:1(13Z)), PE-NMe2(15:0/20:2(11Z,14Z)), PE-NMe2(18:0/18:2(9Z,12Z)), PE-NMe2(18:1(9Z)/16:0), PE-NMe2(18:2(9Z,12Z)/16:0), and PS(15:0/20:3(8Z,11Z,14Z))
These findings collectively indicate that alkaloid metabolites target the central node of the AMPK pathway, providing a multi-target intervention strategy for NAFLD treatment through a dual mechanism of inhibiting lipogenesis while promoting fatty acid oxidation
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