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A 17.5 min long chromatographic program, including a gradient, was applied using the mobile phases H 2 O with 0.1% acetic acid and ten mM ammonium acetate and MeOH with 0.1% acetic acid and ten mM ammonium acetate

This design simulates long-term protective treatment and is a validated model for assessing hepatoprotective agents, and animals were euthanized 24 hours later for liver and blood collection

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Ikesugi, P

In all tissues, the gpoA displayed a significant decrease in their bacterial loads, while the wt gpoA + strain restored the wt infection levels (Fig