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The short plasma half-life (~14 minutes) means peak levels are transient, which is why weekly or biweekly protocols are common in clinical practice
doi: 10.1002/oby.21068, 118 PuhlR
The involvement of miR-25 in the regulation of apoptotic marker genes via downregulation followed by recovery of response upon reconstitution, as well as the EGCG-mediated induction of apoptosis and interference with cell cycle progression (G2/M block), provide evidence that miR-25 may be a regulator of the apoptotic effects created by EGCG in these cells
On the day of assay 2 g per well of the cell membrane was incubated with furimazine (1:1000 dilution from stock) in assay buffer (1 HBSS, 10 mM HEPES, 0.1% (w/v) BSA, 1 P8340 protease inhibitor cocktail, 1 mM DTT and 0.1 mM PMSF, pH 7.4)
Fontana L, Meyer TE, Klein S, Holloszy JO
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