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2.9.3 RT-PCR using SYBR Green Primer testing was conducted using a 25-L reaction mix that contained 12.5 L of the 2x Quanti-Tect PCR SYBR Green Master Mix (Qiagen, Germany), 0.25 L of Revert-Aid Reverse Transcriptase (200 U/L) (Thermo Fisher), 0.5 L of each primer of 20 pmol, 8.25 L of water, and 3 L of RNA template

Dosed to your biology not a protocol

Take the pill when you first wake up, wait 30 minutes, and then start the rest of your day, and you'll be fine

The main goal here would be to measure how much Growth Hormone (GH) the cells release, and how quickly they do it, when exposed to the peptide blend