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Crucially, FOXO4-DRI selectively induced apoptosis in senescent TM3 Leydig cells by causing nuclear exclusion of active Ser15-phospho-p53, leading to a significant increase in apoptosis rate and altering pro-apoptotic (BAX, cleaved-Caspase3 increase) and anti-apoptotic (BCL-2 decrease) protein levels

HPLC and MS/MS validation performed per batch

The effective cost per therapeutic dose likely favors injection when bioavailability discounting is applied

Melatonin secretion starts in infants between 3 and 6 months of age, increases to maximal levels in early childhood, then decreases at puberty onset