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This, in turn, ameliorates oxidative stress, mitochondrial dysfunction, and insulin resistance in the tissues of a non-obese model of type 2 diabetic GK rats

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Western blot analysis The levels of cellular proteins were quantified using Western blot analysis by utilizing primary antibodies: anti--catenin (1:1000), anti-pGSK3 (Ser9, 1:1000), anti- tGSK3 (1:1000), anti-cyclin D1 (1:1000), anti-p-Akt (1:1000), anti-tAkt (1:1000) (all from Cell Signaling Technology, Danvers, MA, USA), or anti-GAPDH (1:5000

Soranna D, Scotti L, Zambon A, et al

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