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B12 injections work by delivering a direct and concentrated dose of vitamin B12, typically in the form of hydroxocobalamin, into the bodys tissues, specifically the muscle (intramuscular)

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After placement on a magnetic stand to remove the buffer, samples were resuspended in antibody buffer (wash buffer with 0.02% digitonin and 2 mM EDTA) and incubated with the TRIM28 or AP2 antibody overnight at 4 C

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