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Contraindications Minoxidil contraindications include hypersensitivity to the drug or vehicle ingredients, pregnancy, breastfeeding, and age under 18

Surgery and spinal cord injury Deeply anesthetized (3% isoflurane, ketamine 50 mg/kg b.w.) rats were subjected to laminectomy at lumbar level L2L3, which corresponds to the sacrocaudal spinal cord (S2-Co1) as described previously [42]

Timelines matching mechanisms: Gene expression changes happen rapidly (hours to days) but take weeks to months producing measurable effects as new proteins accumulate