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10.1186/cc9286 64 DixonB.SmithR.SantamariaJ.OrfordN.WakefieldB.IvesK.et al (2016)

After washing twice with UA as above, 100 L of 50 mM AmBic (50 mM NH 4 HCO 3 in water) was added to the filter unit and the filter was centrifuged at 14,000 g for 10 min