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In vitro experiments confirmed that A 25-35 treatment significantly inhibited the activation of the NRF2/SLC7A11/GPX4 signaling pathway in HT22 cells, upregulated the expression of Fe 2+ , TFR1, ACSL4, MDA, and GSSG, and decreased the expression of FTH1, SOD, and GSH, as well as the GSH/GSSG ratio, resulting in dysregulated neuronal iron metabolism, impaired amino acid antioxidant systems, mitochondrial dysfunction, and ferroptosis, which ultimately promoted neuronal injury
(6) in the passive skin allergy test of the high-stability reduced glutathione for injection, after the guinea pig is excited, the diameter of blue spots of a tested group and the absorbance of leachate have no difference compared with a negative group, and have obvious difference compared with a positive group, which indicates that the high-stability reduced glutathione for injection (with the batch number of YJ110401) does not generate passive skin allergy reaction on the guinea pig
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doi:10.1111/andr.13458 Magnussen LV, Helskov Jorgensen L, Glintborg D, Andersen MS
