Afterwards, the supernatants were collected and the fragments snapped frozen prior to homogenization and protein quantification with the Pierce BCA Protein Assay Kit (Prod #23225, ThermoFisher Scientific, France)
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The GIP receptor component may add additional cardiovascular benefits through effects on endothelial function, though this mechanism is less thoroughly characterized
For a typical subject with type 2 diabetes, clearance was estimated to be 0.0348 L/h [95% confidence interval (CI) 0.03270.0369 L/h], and the central and peripheral volumes were estimated to be 3.59 L (95% CI 3.283.90 L) and 4.10 L (95% CI 3.784.42 L), respectively (i.e
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