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Isolation of PV1 and CAV20 BSO resistant mutants BSO-starved HeLa cells were infected with PV1-wt at a moi (multiplicity of infection) of 0.01 and incubated in the presence of BSO for 24 hr at which time partial CPE was observed

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In the early stage of IR, cells work overload to increase insulin secretion to maintain blood glucose stability, with the compensatory capacity more genetically determined