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ProLuCID data was filtered through DTASelect (version 2.0) to achieve a peptide false-positive rate below 1%

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While our study did not employ organelle-specific ROS probes to pinpoint the precise origin of ROS, the combined evidence- significant total ROS accumulation and the finding that ER stress activation is a prerequisite for mitochondrial apoptosis- strongly suggests the existence of an ER-to-mitochondria signaling axis in NBDHEX-induced cell death