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j , Immunoblot of indicated proteins in HeLa cells treated with erastin (5 M) or indicated doses of RSL-3, an inhibitor of glutathione peroxidase 4 (GPX4), for 24 h
However, like many beneficial compounds in the body, its levels decline significantly with age
In the second step of biosynthesis, glutathione synthetase (GS) adds the final amino acid, glycine, to complete the glutathione molecule
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