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doi: 10.1038/s41577-020-0306-5 156 NishimuraHOkazakiTTanakaYNakataniKHaraMMatsumoriAet al
After incubation, the protein solution was analysed by gel filtration chromatography on a HiLoad 16/60 Superdex 200 prep grade column (GE Healthcare), which was connected to an KTAFPLC system (GE Healthcare)
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Blood clots, DVT and PE are very serious side effects
A single concentration (5 M) or 2-fold serial dilutions of unfused albumin variants (785000 nM) or GLP-1 albumin fusions (1.65000 nM) were injected over the immobilized FcRn at a flow rate of 40 L/min at 25 C
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