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NTS (240) were transferred to U-bottom 96 well assay plates containing 200 l of M199 medium supplemented with 5.5 mM D-glucose, penicillin-streptomycin and 5% heat inactivated fetal bovine serum and incubated at 37 C in a 5% CO 2 incubator overnight

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Complex naturally present in human plasma, studied on matrix remodeling and skin regeneration for 50 years