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After DNA extraction and amplification, the PCR product was loaded on 1% agarose gels (UltraPure Agarose, Invitrogen, Thermo Fisher Scientific), then separated for 30 minutes at 150 V (Owl Easycast B2 Separating System), and the bands were visualized under a blue light transilluminator (UVP GelDoc-It2 Imager)
Jiang W et al
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Cycles of 4-6 weeks on, 2-4 weeks off are standard
Additional mechanistic studies are needed to confirm these assumptions
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