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Perfusion was performed by flushing pre-warmed RPMI containing phenol red and L-glutamine through a 25- and 3/8-gauge needle placed into the aorta attached to Tygon tubing aided by the Masterflex L/S perfusion pump as described 81

Measures to reduce peripheral A protein levels, such as dialysis, plasma exchange, and peripheral administration of the A monoclonal antibody aducanumab, also significantly reduced A levels in brain [40, 41]

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