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There have been some side effects associated with the use of this drug wherein the patient had one of the issues listed below at some point while being on methylene blue

During the in vivo investigation on AR mice, it has been confirmed that treatment with (2 S , 7 S )- O -(2-methyl butanol)-columbianetin reduced the histamine, IgE, and IL-1 levels in serum also reduced the MIP-2 and Intercellular Adhesion Molecule 1 (ICAM-1) protein levels and eosinophils and mast cells in the nasal mucosa tissue and reduced the spleen weight and increased the IFN- levels in the spleen tissue (Nam et al., 2014)

The procedure involves multiple small injections and typically requires 2-6 sessions, spaced a few weeks apart for optimal results

The appearance of the symptoms of trouble breathing and a rash requires prompt medical assistance

This hypoxia, coupled with increased erythropoietin production, can lead to a rise in red cell mass and subsequent iron accumulation in hepatocytes [13]