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This is an expensive, time-consuming, and utterly demoralizing scenario that our team has seen derail promising research projects
Digestion was stopped with 10 mL of ISC basal medium [Advanced DMEM/F-12 with 2mM L-glutamine, 15 mM HEPES, and 1% P/S], followed by centrifugation at 800 g for 3 minutes
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While BAC water is our primary recommendation, other options exist for specific experimental needs
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