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The supernatant was serially diluted and used (in theory, the final concentration of the corresponding E-64 analogs in the mixture was estimated to be less than 100 nM after serial dilution) for the cathepsin B inhibition assay
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For instance, obese individuals experienced an increase in food intake by 18.3 5.3% at a low dose and 32.8 8.7% at a high dose compared to placebo
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