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Following incubation, total RNA was isolated from cells using the TRIzol Plus RNA Purification System (Life Technologies, Carlsbad, CA, USA)

Ozempic, Wegovy and Mounjaro are all administered on a weekly basis through a subcutaneous injection, with the outlier being Mounjaro due to the significantly higher starting dosage (2.5mg compared to 0.5mg for the other two)

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