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For example, often the log ratio of scaled expression data for every array probe is calculated using the following formula: log (Cy 3 BGSS/ Cy5 BGSS), where Cy 3 signal corresponds to the expression of the gene in the clinical sample, and Cy5 signal corresponds to expression of the gene in the reference RNA
If you live in Mexico, Texas, Florida, or a similar area, you can occasionally get away with using a topwater frog during a mild spell
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