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Samples were then washed twice with wash buffer (50 mM HEPES Sodium Salt pH 7.7, 300 mM NaCl, and 25 mM Imidazole) and eluted in elution buffer (50 mM HEPES Sodium Salt pH 7.7, 300 mM NaCl, and 250 mM Imidazole)
The subject is then imaged with appropriate technology such as MRI (when the label is gadolinium) or with a gamma counter (when the label is a gamma emitter)
Wrap the remaining string around the end of the stick
One thing to note when choosing rod length is that there is typically a correlation between the angler's height and their preferred rod length
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