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An optimized workflow for the analysis of metabolic fluxes in cancer spheroids using Seahorse technology

harzianum s colony diameter decreased gradually by increasing Pb (II) concentration (Fig

Superoxide anion (O 2 - ), hydroxyl (OH ), alkoxy radicals (RO ), peroxyl radicals (ROO ), nitric oxide radical (NO ), nitrogen dioxide (NO 2 ), and carbonate radical (CO 3 - ) are the few examples of radical ROS whereas non-radical ROS include singlet oxygen ( 1 O 2 ), hydrogen peroxide (H 2 O 2 ), hypochlorous acid (HOCl), and peroxynitrite (ONOO - ) species which can readily trigger free radical reactions in organisms [23, 24]

The PLS-5 provides a comprehensive assessment of children's receptive and expressive vocabulary and is administrated by a trained assessor ( The developmental status of each participant was measured using the Mullen Scales of Early Learning (MSEL) administered by a trained assessor and the Vineland Adaptive Behavior Scale, 2nd edition (VABS-II) completed by parent interview