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For mass spectrometric analysis, sample processing was further proceeded with reduction and alkylation, followed by acetone precipitation and tryptic digestion
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Additionally, although the thiolactone form has not been unequivocally identified in vivo , homocysteine could undergo intracellular cyclization into homocysteine thiolactone, which could be a major by-product of impaired homocysteine metabolism (Jakubowski, 1997
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Blood 92(9):3090-7, 1998
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