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After 48 h of transfection, cells were harvested and transfection efficiency was quantified by qRT-PCR

GSSCys and HEDS oxidoreductase assays Steady-state kinetics of wild type and mutant ScGrx7 and PfGrx in the GSSCys and HEDS assays were determined spectrophotometrically by monitoring the consumption of NADPH at 340 nm and 25 C using a thermostated Jasco V-650 UV/vis spectrophotometer 11,12,18

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Fact: Most standard glutathione is destroyed by digestion