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Lipid peroxidation was measured with using Bodipy 581/591 C11 (Fisher Scientific D3861) at 1 M following the manufacturers protocol

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AFS can result in imbalances in the different circuits, including the detoxification system, and result in the body being unable to effectively remove waste products and toxins

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Quantitative reverse-transcription PCR (qRT-PCR) Total RNA extraction, cDNA synthesis, quality and quantity analysis, and qRT-PCR were performed as described previously (Zhang et al