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Differences were analyzed by one-way or two-way analysis of variance (ANOVA) with Bonferroni post-test, Student t test, multiple t-test with Holm-Sidak method, MannWhitney test, or Mantel-Cox test, as appropriate

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however, weight gain was not tested in these studies

PD-L1 is a novel direct target of HIF-1, and its blockade under hypoxia enhanced MDSC-mediated T cell activation