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Quantitative real-time PCR RNA was purified by using ReliaPrep RNA (Promega, WI, USA) and reverse transcription was performed using 1.5 g total RNA as template with the ImProm-II Reverse Transcription System (Promega, WI, USA)

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Some of the main ways in which our body uses B12 include supporting red blood cell distribution, cognitive function, and neurological and cardiovascular health