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After administration of glucose, blood glucose levels were measured from minor tail pricks at desired time intervals (0, 15, 30, 60, 90, and 120 minutes) using a glucometer (CONTOUR NEXT, Ascensia Diabetes Care)

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Preclinical research comes first, followed by Phase 1 safety trials, Phase 2 efficacy trials, Phase 3 registration trials, NDA submission, FDA review, and finally approval or rejection

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