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Two hundred microliters of loading buffer were added and beads were mixed for 2 min followed by two washes with wash buffer 1 (80% acetonitrile, 1% TFA) and once with wash buffer 2 (10% acetonitrile, 0.2% TFA)
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doi:10.1016/j.stemcr.2021.07.003 106
Gapdh was used as an endogenous housekeeping gene (reference gene) and the values were normalized to it
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