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Nanoprobe-based GSH imaging relies on signal-generating reactions triggered by glutathione, and effective tumor visualization requires that these signals clearly differentiate malignant tissue from normal tissue
For cell proliferation assays with fold change in cell number, cells were cultured in replicates of 3 in 96-well plates at 1000 (suspension) or 150 (adherent) cells per well in 200 uL RPMI or DMEM base media under the conditions described in each experiment, and a separate group of 3 wells was also plated for each cell line with no treatment for an initial time point
L-ascorbic acid (vitamin C), L-glutamine, magnesium citrate (magnesium), potassium citrate (potassium), concentrated beetroot juice, sweeteners: sucralose, acesulfame K
Author contributions ZZ: Writing original draft, Formal analysis
Produced in small batches under controlled conditions, each lot is supported by verified COAs to ensure purity, consistency, and quality for research applications
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