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To this end, BALB/c mice, which are genetically deficient in endogenous melatonin production (28), were intranasally challenged with 25 g Alternaria alternata (AA) or PBS daily for four days, with or without concurrent intraperitoneal melatonin treatment using established protocols described previously (18, 2931) ( Figure 1A )
Reduced glutathione (in 100 mM K 2 HPO 4 /KH 2 PO 4 buffer, pH 7.0) was automatically injected to the indicated final concentration using the built-in injectors
R., Blackburn, W., Orem, I., and Hughes, J
PEG incorporation extends circulation, reduces uptake by liver and spleen, and shows no toxicity (Gregoriadis 1995
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