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For mitochondrial ROS detection, the cells were stained with 5 g/mL MitoSox dye (Thermo Scientific, Waltham, MA, USA) at 37 C for 15 min, followed by three PBS washes

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Retatrutide alone risks 20-30% muscle loss in total weight reduction, but combining with 1.6g protein per kg body weight daily and resistance training twice weekly preserves up to 70% more lean mass

Rotate sites with each administration