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After centrifugation (17,000 g , 20 min, 4 C), all the supernatants were analyzed by LC-HRMS

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There is no wrong amount of water, only wrong math

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Use the formula: V draw = D anchor V water M anchor D anchor is your target dose in mg, V water is the BAC water volume you reconstitute with in mL, and M anchor is the anchor compound mass in the vial in mg