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The pellets were finally resuspended in TE buffer
In addition, age dependent accretion of dysfunctional mitochondria elevates stress level, which is exhibited by high levels oxidized products such as protein carbonyls and lipid peroxides 42
Open and reconstitute vial 2 only when you begin day 6
In all, 40 L of each normalized sample was collected as input and was boiled at 95 C for 5 min after 8 L of six times loading dye was added to each input sample
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