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Most patients report only a slight pinch during the IV insertion

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Cryo-EM grid preparation and data acquisition The purified DDB1-DDA1-DET1 complex was prepared at a concentration of 0.2 mg/ml and applied to glow-discharged UltrAuFoil (1.2/1.3, 300 mesh) grid, with an incubation time of 10 sec