* Correspondence: Rocco Mollace [email protected] Roberta Macr [email protected] These authors have contributed equally to this work Disclaimer All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers
Quantification of GSH using Ellmans assay Cells were harvested and lysed with 0.1% Triton-X and 0.6% sulfosalicylic acid in 0.1 M potassium phosphate buffer with 5 mM EDTA at pH 7.5 to afford protein free lysate
In melanocytes 4-HNE, through adduct formation, alters protein function, contributing to oxidative stress-induced cellular dysfunction, which mainly concerns on the functioning of cellular antioxidant system, acceleration of protein degradation, inhibition of melanocyte proliferation, photoaging processes, but also disruption of the intercellular interactions between melanocytes and keratinocytes 101,102,103
Both promote angiogenesis through different pathways, creating redundant and amplified blood vessel formation
Metformin (Sigma-Aldrich, D150959-5G) was prepared at a concentration of 200 mg/kg and administered via intragastric administration once daily over the eight-week program