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Additionally, established procedures help support dependable batch-to-batch performance and reliable research material preparation
Following treatment, the medium was removed, cells were washed 2 times with warm PBS, and fixed with 4% formaldehyde for 20 min
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although both shorter and longer linkers have been reported, it still seems empirical and optimization is required for each pair of ligase and target protein, since it is likely that optimal linker identity will vary for different proteins targeted
