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Glucagon-like peptide-1 receptor agonists (GLP-1 RAs), initially developed for type 2 diabetes management, have demonstrated remarkable efficacy in addressing the multifactorial pathogenesis of MASLD/MASH [7-10]
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To prepare the long single-stranded editing donor DNA (lssDNA), the 1.5 kb insert was amplified from slc25a39-flox-E2-Puc57 and processed to lssDNA using exonucleolytic hydrolysis of its 5-phosphorylated DNA strand as described previously 44
