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The annulus fibrosus forms the outer ring, a complex series of fibrous layers made primarily of type I collagen arranged in alternating diagonal patterns
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The transfer vectors were co-transfected with flash BAC ULTRA (FBU) viral DNA (OET) to generate recombinant baculoviruses FBU EEHV-EE2 and FBU EEHV- MCP 216-1349aa in Sf9 insect cells (Fisher Scientific, Loughborough, UK)
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