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If you are losing more than 2 percent of body weight per week over more than two consecutive weeks, that is a hold signal too, because the deficit is likely too aggressive for lean mass preservation regardless of how motivated you feel

For CDDO-methyl experiments, WT mice without AAV-TBG-Cre injections were injected intraperitoneally with 5 mg/kg of either vehicle (10%DMSO in 90% corn oil) or CDDO-methyl (MedChem Express, HY-13324) for 4 days

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