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Briefly, to verify the targeted binding between miR-29b-3p and the GGCT gene, a 2000-bp DNA fragment (derived from the transcription start site of the GGCT gene) was inserted into the pmir-GLO-Basic vector to construct the wild-type pmir-GLO-GGCT-3 UTR reporter plasmid (wt) and the mutant pmir-GLO-GGCT-3 UTR reporter plasmid (mut)
Protocols describe swirling gently rather than shaking
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10.1111/apha.14159 108 ZhangY.ZouL.LiX.GuoL.HuB.YeH.et al (2024b)
Weeks 8-12 : More significant improvements in deeper wrinkles, continued collagen density increase, improved skin thickness (measured via ultrasound in clinical settings), and sustained reduction in age-related skin laxity
