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Collagen and elastin, the proteins responsible for skin tone and firmness, require synthesis stimulation that the body may not provide at an adequate rate on its own, particularly when caloric intake is suppressed

Periodic acid-Schiff (PAS) staining and scanning electron microscopy (SEM) imaging After cultivation for 18 h, biopsies were collected and fixed for i) PAS staining in 7.5% formaldehyde (SAV liquid production GmbH, Germany), and ii) SEM in Karnovskys fixative (2% paraformaldehyde, 2.5% glutaraldehyde in 0.1 M phosphate buffer pH 7.4

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Its structure is the cyclic peptide Ac-Nle-cyclo[Asp-His-D-Phe-Arg-Trp-Lys]-NH