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Is it necessary to filter the solution after reconstitution
The bacterial cells were harvested, resuspended in binding buffer (20 mM sodium phosphate, pH 7.4, containing 500 mM NaCl and 20 mM imidazole), and lysed by treatment as above, followed by sonication
[1] When administered, these medications stimulate insulin release from the pancreas in response to elevated blood glucose levels, thereby lowering blood sugar
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