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RNA extraction, library construction, and sequencing According to the manufacturers instructions, a TRIzol reagent kit (Invitrogen, Carlsbad, CA, USA) was used to extract total RNA

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Palmitate oxidation rates were detected at 28 C using two media as already described 59 , the first media allowing both mitochondrial and peroxisomal -oxidation to occur, and the second one allowing peroxisomal -oxidation only