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Cells from all replicates were combined for further analyses

To assess the half maximal inhibitory concentration (IC50) of FOXO4-DRI on the serum limitation-induced senescent fibroblast and the non-senescent control fibroblast, the fibroblast viability in the 2% FBS induced senescent fibroblast and 10% FBS cultured non-senescent control fibroblast with increasing concentrations of FOXO4-DRI was detected, and the cell viability curves were showed in Supplementary Fig

What a COA (Certificate of Analysis) Should Include Identity Confirmation Mass spectrometry confirming molecular weight ~419.4 Da (molecular formula C22H21N5O3) NMR or LC-MS/MS structural confirmation for a small molecule (peptide vendors often skip this small-molecule identity confirmation is non-negotiable here) Purity Testing HPLC purity 98%+ minimum Single dominant peak on the HPLC chromatogram Contamination Testing Residual solvent panel (DMSO, ethanol, acetone all common in small-molecule synthesis) Heavy metals screen Endotoxin testing if the product is positioned for injectable use How to Verify a COA Is Legitimate Match lot number to batch