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Cells were suspended in Seahorse XF RPMI medium (200 ul/well, Agilent, 103576-100) supplemented with 10 mM glucose, 2 mM glutamine, and 1 mM sodium pyruvate
This concept was extended when highly powered single-cell (sc) transcriptomics studies allowed a more detailed characterization of endothelial cell heterogeneity in homeostasis and disease
& Edison, P
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