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Taking into account the amyloid -protein oligomer paradigm in the etiology of AD, as early and pathognomonic predictors of the disease, some interesting works reported the existence of several challenges that must be faced during A oligomer detection in vitro as well as ex vivo through different techniques of analysis, including SDS-PAGE rather than surface enhanced laser desorption/ionization time-of-flight mass spectrometry (MS) or size exclusion chromatography, which may give rise to misleading data (Marina et al., 2003

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